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KMID : 1098420120200030190
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Korean Journal of Medicinal Crop Science
2012 Volume.20 No. 3 p.190 ~ p.194
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In Vitro Micropropagation of Chinese Yam (Dioscorea opposita Thunb.) through the Culture of Micro-tuber Sections and by Addition of Liquid Medium
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Kim Young-Ho
Lim Soon-Taek
Han Bong-Hee
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Abstract
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Shoot tips of chinese yam (Dioscorea opposita Thunb.) were cultured on MS medium containing 0.5 ㎎/L BA to produce micro-tubers in vitro. To stimulate the formation of shoots and micro-tubers, and produce large micro-tubers, the sections of micro-tubers were cultured on MS media with BA and IAA. The shoot multiplication, and the micro-tuber formation and growth were very effective on the media containing 2.0 ㎎/L BA and 0.5~1.0 ㎎/L IAA. Sucrose added to MS medium with 2.0 ㎎/L BA and 0.5 ㎎/L IAA to stimulate more micro-tuber growth. The medium added 50 g/L sucrose was very effective in the increase of plant fresh weight and micro-tuber growth. After 4 weeks’ culture of micro-tuber sections on the medium with 2.0 ㎎/L BA, 0.5 ㎎/L IAA and 50 g/L sucrose, the liquid media were added into the same vessels. The micro-tuber growth was stimulated remarkably by the addition of liquid medium. The addition of 25 ㎖ liquid medium containing 10 g/L activated charcoal, 3x MS salts and 250 g/L sucrose was the most effective in micro-tuber growth.
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KEYWORD
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Chinese Yam, Dioscorea opposita Thunb., Liquid Medium, Micropropagation
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